Title of article
Micropropagation of Asam Karanda (Carissa carandas Linn)
Author/Authors
Hasmah, Siti Nor Universiti Sains Malaysia - School of Biological Sciences - Plant Tissue and Cell Culture Laboratory, Malaysia , Bhatt, Arvind Universiti Sains Malaysia - School of Biological Sciences - Plant Tissue and Cell Culture Laboratory, Malaysia , Keng, Chan Lai Universiti Sains Malaysia - School of Biological Sciences - Plant Tissue and Cell Culture Laboratory, Malaysia
From page
89
To page
98
Abstract
An efficient micropropagation protocol was developed for C. carandas using nodal segment as the explants. Murashige and Skoog medium (MS), supplemented with 2.0 – 4.0 mg/L Benzylaminopurine (BA), induced an average of 3.6 - 5.5 shoots/explants but the number of multiple shoots formed was not significantly different. However, the addition of IBA combined with BA had shown a reducing trend in the number of shoot being formed. The MS medium supplemented with 2.0 mg/L 3-Indolebutyric acid (IBA) was found to be effective for rooting of micro-shoots. After the acclimatization process, 84% ± 4.3% of the in vitro plantlets survived but there was a reduction in the number of survived plantlets (59 ± 3.7%) after 4 weeks of incubation in the green house condition.
Keywords
Acclimatization , nodal segment , shoot multiplication , root formation
Journal title
Pertanika Journal of Tropical Agricultural Science (JTAS)
Journal title
Pertanika Journal of Tropical Agricultural Science (JTAS)
Record number
2652464
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