Title of article
Molecular and Physiological Detection of Cyst Nematodes on Potato During Plant Nematode Interactions
Author/Authors
elkobrosy, dina h. city of scientific research and technological applications - arid lands cultivation research institute - plant protection and biomolecular diagnosis department, Alexandria, Egypt , aseel, dalia g. city of scientific research and technological applications - arid lands cultivation research institute - plant protection and biomolecular diagnosis department, Alexandria, Egypt , abdelsalam, nader r. alexandria university - faculty of agriculture (saba basha) - agricultural botany department, Alexandria, Egypt , el-saedy, mohamed a. alexandria university - faculty of agriculture (elshatby) - plant pathology department, Alexandria, Egypt , shama, saad alexandria university - faculty of agriculture (saba basha) - agricultural botany department, Alexandria, Egypt , hafez, elsayed e. city of scientific research and technological applications - arid lands cultivation research institute - plant protection and biomolecular diagnosis department, Alexandria, Egypt
From page
478
To page
481
Abstract
Cyst nematodes involve over than 90 known species into six genera, G. rostochiensis and G. pallida are most important pathogens of potato crops where their economically a yield losses. The aim of present investigation is characterization the cyst nematode species (G. rostochiensis) using molecular techniques during plant-nematode interaction. The molecular identification of cyst nematode was carried out using ITS PCR. The results of ITS-PCR showed two bands with different molecular sizes 238 bp and 370 bp. The metabolic activation of potato leaves infected with cyst nematodes was detected. The results confirmed that, G.rostochiensis significantly elevated the contents of shoots lipid peroxidation (MDA) as compared to the untreated healthy plants.
Keywords
Cyst nematodes , ITS PCR , potato
Journal title
alexandria science exchange journal
Journal title
alexandria science exchange journal
Record number
2656648
Link To Document