Haemopoietic Function and Flowcytometry of Pronephric Kidney in Clarias batrachus L. under the Impact of Organophosphate- Sumidon 40

The impact of different doses of SUMIDON-40, an organophaphate pesticide on the haemopoiesis of Clarias batrachus L. (Clariidae) was tested. The small lymphoid haemoblast decreased and consequently the young erythrocytes increased significantly in both sublethal and LC50 dose treated fish. The percentage of mature reticulocyte increased significantly in LC50 dose treated group. The erythropoietic efficiency was increased significantly in fish exposed to sublethal dose and decreased in fish exposed to LC50 dose. Among leucocytes, the percentage of neutrophil increased significantly in both treatment groups and the percentage of macrophage increased only in LC50 dose treated group. The overall leukopoietic efficiency, however, was increased significantly in both treatment groups. Flowcytometric analysis of cell cycle in pronephric kidney cells confirmed that the cell death was increased and DNA synthesis was significantly decreased with increasing dose of pesticides. It is concluded that habitat deterioration caused by agrochemical impedes haemopoiesis in this species resulting to reductionof endurance levels to pollution.