Title of article
Role of Pili and Polysaccharide in adherence of Pseudomonas aeruginosa to mammalian epithelial cells
Author/Authors
Hamza, Subhi J. Baghdad University - Collage of Science - Dept of Biotechnology, Iraq , Al-Zubaidy, Sumuiyya H. Ministry of Trades - Department Of Evaluation and QualityControl, Iraq , Al-Amir, Lina A.K. University of Baghdad - College of Science - Department of Biology, Iraq
Pages
7
From page
111
To page
117
Abstract
Adherence capability of Pseudomonas aeruginosa isolated from Iraqi patients was tested in accordance to their capability to adhere to epithelial cells. A range of adherence ability was found: The highest capacity with mean number of adhering bacteria of (13.5) was noticed. A sparse content or alginate was detected in these isolates analysis of pilin protein isolated from cultures exposed to ciprofloxacin antibiotic at sub MIC, level using polyacrylamide gel electrophoresis indicated that treatment of cells with 3/4 MIC show highest activity as compared with others. Results of protein profile followed the same trend of that in adhesion experiment which allows hypothesizing that these isolates are piliated, not mucous.
Keywords
Polysaccharide , adherence , Pseudomonas aeruginosa , mammalian epithelial cells
Journal title
Journal Of Biotechnology Research Center
Serial Year
2010
Journal title
Journal Of Biotechnology Research Center
Record number
2673985
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