Title of article
Antibiotic resistance among Escherichia coli urinary isolates and their susceptibility to clove essential oil
Author/Authors
dąbrowski, michał medical university of łódź - department of allergology and respiratory rehabilitation, Poland , sienkiewicz, monika medical university of łódź - department of allergology and respiratory rehabilitation, Poland , zielińska-bliźniewska, hanna medical university of łódź - department of allergology and respiratory rehabilitation, Poland , dąbrowska, marta district hospital in garwolin - department of gynecology and obstetrics, Poland , dąbrowska, marta medical university of łódź - department of allergology and respiratory rehabilitation, poland , seredyńska, małgorzata military medical institute - clinic of anesthesiology and intensive therapy, Poland , kochan, ewa medical university of łódź - pharmaceutical biotechnology department, poland
From page
41
To page
48
Abstract
Escherichia coli is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium, which is a primary cause of urinary tract infections. Resistance to antibiotics has become a particular problem in recent decades. Consequently, there is an unmet need for new therapeutic options. It has been observed that essential oils have bactericidal effects. The antimicrobial susceptibility testing for Escherichia coli isolates obtained from urine of patients with urinary tract infections was determined via disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2015). Essential oil from clove – Syzygium aromaticum (L.) Merill et L.M. (Myrtaceae) was analyzed by GC-FID-MS. Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) were detected by using the micro-dilution broth method. Escherichia coli clinical isolates are characterized by high resistance to ampicillin, amoxicillin with clavulanic acid, norloxacin, trimethoprim/sulfamethoxazole, tetracycline, tobramycin and ticarcillin. Clove oil possesses strong inhibiting and killing properties against E. coli isolates, among them the ones resistant to recommended antibiotics. The results of this study highlight the need for testing the eficacy of new agents to inactivate bacteria in clinical settings.
Keywords
Escherichia coli , clove oil , urinary tract infections
Journal title
Annales Universitatis Mariae Curie-Sklodowska Sectio C Biologia
Journal title
Annales Universitatis Mariae Curie-Sklodowska Sectio C Biologia
Record number
2677185
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