Extraction and Purification of Vibrio Cholerae Enterotoxin and Study its Cytopathic Effect on Some Mice Organs

Vibrio cholerae enterotoxin was extracted by cooling centrifugation and filtration with milipore filter (0.22um) and was purified by using Sephacryl –S- 6 gel filtration,the content of protein was estimated . The results showed protein concentration was 28.5 microgram/ml,the present of enterotoxin was detected by infant suckling mouse method..The cytopathic effect of enterotoxin was studied by injecting a number of mice with purified enterotoxin, It was found caused shortening the villi of the intestine at concentration 55 and 45 ug /ml of purified enterotoxin, while the effect on liver showed degenerative change with necrosis at 55 ug/ml of enterotoxin and caused necrosis and infiltration of inflammatory cells at 45 ug /ml, the effect on spleen tissue was represented by widening of white pulp, and reduction or disappearance of red pulp also inflammatory cells wre found i.e. lymphocyte and hypoplasia of white pulp at the two concentrations of the enterotoxin