Isolation and Identification of cloves oil from eugenia caryophyllata using Ultrasonic extraction technique

This study is designed to isolate and identify of essential oil eugenol from cloves, an important medical plant used in various pharmaceutical formulations .The Isolation process is carried out by Ultrasonic bath technique and simple distillation, with water and · extraction with various organic solvents. Optimum organic extr11ctant and optimum pH for both techniques extraction are determined. The oil was determined spectrometrically at 640nm with Folin- Ciocalteau reagent, the maximum extraction yield was estimated in ultrasonic extraction technique. The collected oil is· identified via Thin Layer Chromatography (TLC) using a mixture of Ethylacetate: tolune (I :9) as chromatographic eluent. Spectroscopic studies Ultraviolet- Visible (UV-Visible) spectrometry and Infra-Red (IR} spectrometry are also conducted for identification eugenol oil from cloves.