Title of article
The Interaction of Hemoglobin With Sodium Dodecyl Sulfate in Presence of Ascorbic Acid
Author/Authors
Mirzajani ، Radnoosh Department of Chemistry - Islamic Azad University, Rasht Branch , Mirzajani ، Ebrahim Department of Biochemistry - Faculty of Medicine - Guilan University of Medical Sciences , Ebrahimi-Najafabadi ، Heshmatollah Department of Medicinal Chemistry - School of Pharmacy - Guilan University of Medical Sciences
From page
21
To page
26
Abstract
Background: Hemoglobin (Hb), oxygen, carbon dioxide, and electron transporter of the body, may enter to an oxidation process that can convert oxyhemoglobin (oxyHb) to methemoglobin (metHb) and hemichrome. Surfactants can facilitate oxidation process that may accumulate hemichrome in red blood cells. Methods: In the present study, the interaction of purified Hb with sodium dodecyl sulfate (SDS, 0-5 mM) and ascorbic acid (AA, 0-5 mM) was evaluated by UV-Vis spectroscopy and multivariate curve resolution techniques. Results: Reconstructed spectral and concentration profiles showed three forms of Hb name as oxyHb, metHb, and hemichrome with lack of fit values less than 1.85%. AA hindered the oxidation process of Hb. Conclusion: A decrease in critical micelle concentration of SDS in the presence of AA and interaction of AA with hydrogen peroxide, which is produced during the interaction of Hb with SDS, are two reasons for diminution in the oxidation process of Hb when accompanied with AA
Keywords
Hemoglobin , Sodium dodecyl sulfate , Ascorbic acid , Peroxide , Multivariate curve resolution , Chemometrics
Journal title
Avicenna Journal of Medical Biochemistry
Journal title
Avicenna Journal of Medical Biochemistry
Record number
2695792
Link To Document