Determination of Dunaliella Salina Phenolic Compounds in Laboratory Different Conditions

Phenols are important natural compounds with antioxidant and other biological properties. Phenolic compounds have great advantages in food, chemistries, pharmaceutical and medical industries. Previous studies revealed that many phenolic compounds effectively scavenge hydroxyl and peroxyl radicals and prevent fats oxidation. The aim of this study was to optimize the laboratory different conditions including salinity (1, 3 and 5 M), light intensity (35, 127.5 and 220 μmol) and nitrogen source (200, 500 and 800 mmol/l) on the phenolic content of the algae. Therefore, 15 treatments were designed according to the Box-Behnken response surface methodology in 16 mini tabs. The results showed that laboratory different conditions (salinity, light intensity, and nitrogen content) had significant effect on the number of phenolic compounds in Donaliella salina. The highest amount of phenol, 0.43 gallic acid/g, was obtained at light intensity of 220 μmol photon, salinity of 3 M and nitrate concentration of 200 μmol/L. By optimizing the culture medium of Dunaliella salina, optimal light radiation conditions of 178 μmol photon, salinity of 3.26 M and nitrogen concentration of 327 μmol/L the number of phenolic compounds could be increased (0.46 mg gallic acid/g).