In Vivo Antioxidant Activity of Ruta montana L. Extracts

The aim of this study is to evaluate antioxidant activities of Ruta montana L. extracts. To do this, An in vivo activity was carried out on rats treated with aqueous extract (AqE) at doses of 100 and 300mg/Kg bw and methanolic extract (CrE) at a dose of 100 mg/Kg for 21 consecutive days, and one group was treated with vitamin C (Vit C 50 mg/kg) as a standard drug. The antioxidant activities of aqueous extract and methanolic extract in vivo were estimated using the antioxidant plasma capacity (APC), 2, 2-diphenyl-2-picrylhydrazyl hydrate (DPPH) and reducing power tests. All extracts did not show any significant change. The activity of catalase (CAT), the level of MDA and GSH were evaluated in liver and kidney homogenate. The results showed an increase of CAT activity in the groups treated with AqE (300mg/kg) and CrE (100mg/kg) by 30.76% and 32.07% for liver tissue and 24.48% and 32.11% for kidney tissue, respectively. The GSH levels were increased by 18.57%, 22.97% for liver tissue and 12.3%, 37.36% for kidney tissue, respectively. However, the level of MDA was decreased for the treated groups with AqE (300mg/kg) and CrE (100mg/kg). These results suggest that Ruta montana L. extracts have active substances contributing to the increase of antioxidant potential.