The interactive effect of berberine chloride and exercise rehabilitation on the lung tissue apoptosis and oxidative stress biomarkers in rats exposed todiazinon

Introduction: A serious complication of diazinon is the occurrence of oxidative stress in the respiratory system. The combination of medication and exercise rehabilitation has not been studied. The aim of this study was to assess the effects of berberine chloride alongside resistance training on apoptosis and oxidative stress markers of lung tissue in male rats exposed to diazinon. Methods: Forty-eight adult male Wistar rats were divided into 1: resistance exercise + berberine chloride dose 2mg/kg + diazinon; 2: resistance exercise + berberine chloride dose 15mg/kg + diazinon; 3: toxic (diazinon 1.5 mg/kg); 4: resistance training + diazinon; 5: berberine chloride dose 2mg/kg + diazinon; 6: berberine chloride dose 15mg/kg + diazinon; 7: intact control and 8: normal saline. To induce oxidative stress, diazinon was injected intraperitoneally 1.5mg/kg. Berberine chloride was used as 2 and 15mg/kg through intraperitoneal injection for 4 weeks. Resistance training was conducted 3 sessions/week for 4 weeks including climbing a vertical ladder. Lung tissue was exposed to evaluate pathohistological test, apoptosis and oxidative stress markers. Results: Berberine chloride and exercise had a significant effect on decreasing ROS, MDA, caspase-3, and increasing GSH level, but no effect on the 8-OHDG. The exercise alone has no significant effect on ROS, MDA, 8-OHDG, caspase-3 and GSH. Conclusion: Berberine chloride alongside sport rehabilitation should be used as an effective treatment on lung apoptosis and oxidative stress with acute poisoning of diazinon. The most important mechanism is the effect of improving oxidative defense and anti-inflammatory effects.