The effects of subgingival application of ozonated olive oil gel in patient with localized aggressive periodontitis. A clinical and bacteriological study

This study evaluates the effect of subgingival application of ozonated olive oil gel as an adjunct to scaling and root planing (SRP) in aggressive periodontitis. Material methods: Thirty patients were randomly selected and equally divided into: Group I received SRP only, group II received SRP and ozonated olive oil gel (Oxactiv). Subgingival application of ozone gel was performed following initial SRP and at 7, 14 and 21 days. Clinical measurements included pocket depth (PD), plaque index (PI), gingival index (GI), bleeding on probing (BOP) and clinical attachment level (CAL). Real time PCR was carried out to determine the effect of the treatment on both Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg). Clinical measurements and Plaque samples for PCR were recorded at baseline, one, three and six months after treatment. Results: The results showed improvement in all clinical parameters in (group II) which was maintained up to six months (P 0.05). However, this improvement was best following one month but gradually decrease at 3 and 6 months. Whereas SRP alone resulted in a significant improvement only up to one month for BOP, PPD and CAL parameters and up to three months for the PI and GI scores as compared to baseline values. Microbiological results: Revealed significant reduction of the mean Pg and Aa DNA copies at 1 and 3 months for (groupII), whereas group I resulted in slight reduction up to 1 month only followed by gradual increase reaching baseline values. There was no significant difference between groups at three and six months regarding Pg DNA copies. There was a significant difference between groups at the one and three months periods in term of number of Aa copies (P 0.001, P 0.05 respectively). Conclusion: The study concluded that (Oxactiv) gel could be a promising adjunct to SRP in the treatment of aggressive periodontitis.