Isolation and Characterization of Neoduline from the Rhizome of Dolichos pachyrhizus Harm

Dolichos is a genus of twining plants found in both the Northern and Southern hemispheres, with the most frequently grown and used species being Dolichos biflorus and Dolichos lablab. Antimicrobial, antioxidant, anticancer, anti-schistosomal, and anti-inflammatory properties have been reported in various parts of the Dolichos species, including the leaves, roots, bark, and stem. The rhizome of Dolichos pachyrhizus was cut, air-dried at ambient temperature, and macerated with n-hexane, dichloromethane, ethyl acetate, and methanol for 72 hours each using a conventional maceration procedure, with regular shaking at intervals. The mixture was decanted, filtered, and concentrated at 40 °C using a rotavapor to yield crude n-hexane, dichloromethane, ethyl acetate, and methanol extracts (R110). To obtain the pure isolate, a methanol crude extract of the rhizome of Dolichos pachyrhizus (D. pachyrhizus) was chromatographed on a silica gel column using various eluents. The preliminary phytochemical investigation of the methanolic rhizome extract of Dolichos pachyrhizus revealed the presence of seven (7) different phytochemicals, including; - alkaloids, flavonoids, glycosides, phenols, tannins, saponins and steroids. The structure of the pure isolate was successfully identified, characterized and confirmed using spectroscopic techniques such as; - Fourier transform spectroscopy (FT-IR), Gas chromatography-mass spectrometry (GC-MS) and Nuclear magnetic resonance (NMR) be Neoduline.