Protective Role of αPinene in CuprizoneInduced Multiple Sclerosis in Mice

Introduction: It is clamied that αpinene has properties against Multiple Sclerosis (MS) which is known as demyelination of the neurons. Given that, the aim of this study was to investigate the effect of αpinene on Cuprizoneinduced (CPZ) MS. Materials and Methods: A total of forty C57BL/6 mice were allocated to 4 groups. Mice in group 1 (control) were treated with a normal diet. In group 2, CPZinduced demyelination was done by chew palate containing .2% (w/w) CPZ for 5 weeks. In group 3, a normal diet was provided and mice were injected with 𝛼pinene (1 mg/kg; i.p.) 3 times a week for 5 weeks. In group 4, mice were fed with the CPZ containing diet and injected with 𝛼pinene (1 mg/kg; i.p.) three times a week for 5 weeks. At the end of the study, reflexive motor behavior and depressive like behavior tests were performed. Additionally, serum antioxidant activity was determined. Results: Results show that the CPZ had an adverse effect on reflexive motor behavior tests (P .05) and coadministration of the CPZ+𝛼pinene diminished the adverse effect of the CPZ on the reflexive motor behavior tests (P .05). Moreover, CPZ significantly amplified immobility time (P .05) and coadministration of the CPZ+𝛼pinene reduced the adverse effect of the CPZ on depressivelike behavior tests (P .05). CPZ significantly increased malondialdehyde (MDA) and decreased glutathione peroxidase (GPx), superoxide dismutase (SOD) and total antioxidant status (TAS) and also these effects were reversed by αpinene (P .05). The data indicate that coadministration of the CPZ+𝛼pinene significantly improved the adverse effect of the CPZ on serum antioxidants (P .05).