An In Vivo Synergistic Research on the Assessment of the Phytocompound Capped Silver Nanoparticles from Raphanus sativus Leaf Extract and Curcuma longa Against UTI-Causing E.coli.

Recently, green synthesis of nanoparticles using plant has attracted much attention due to the environmental and economic benefits. The present work demonstrates an eco-friendly and low-cost method to study the antibacterial activity of the silver nanoparticles [AgNPs] biosynthesized from phenolic compounds of plant extracts (Raphanus sativus and Curcuma longa). Using GCMS and 1H NMR spectroscopy analyses, the presence of phenolic compounds and alcohol groups of long-chain were detected. Polyphenol compound such as catechol and phenolic derivative such as curcumin were capped with silver nanoparticles and proved by SEM analysis. However, the antibacterial potential of these green synthesized phytochemical capped AgNPs was further explained in in vivo studies. Albino mouse was induced with multidrug-resistant E.coli and was treated with Ciprofloxacin as a conventional drug for the standard groups and with synthesized catechol-curcumin capped silver nanoparticles for the test groups. Histopathology of albino mice kidney and bladder was performed after dosing the mice with specific concentrations of the nanoparticles for 3, 7, and 14 days. Photomicrographs of the kidney and bladder histology revealed cell regeneration, normal renal cortex, and glomerular tufts in the mice exposed to the nanoparticles.