PCR Based Genetic Diversity in Common Bean (Phaseolus vulgaris) Landraces Collected from Malakand, Division, Khyber Pakhtunkhwa, Pakistan

Thirteen SSR markers were used for sixty local common beans landraces. Among all markers, only one was monomorphic, while the remaining were polymorphic. At average 2 alleles per locus were identified. The size of all alleles ranged from 210bp to 630 bp. Major allelic frequency fluctuated from 54.0% to 96.0%. An overall, the total No. of alleles per locus extended from 22 (PVBR 20) to 49 PvM053. Similarly, the frequency values oscillated from 0.06 of PVBR 20 to 0.14 of PvM053. The PIC values were ranged from 0.295 of PVBR 20 to 0.831 of PvM053. The Two Way Cluster dendrogram was conducted for 12 polymorphic SSR markers, which disseminated all common bean lines into two major linkages. Lineage I split at 50% similarity index into 5 clusters and sub clusters. On the same way lineage II split into two clusters at 19 % similarity index. The Genetic similarity (GS) based on SSR markers between pair of common bean lines showed high degree of dissimilarity in the genetic makeup ranging from 0.91 to 0.11. All the landraces of common bean collected from Malakand divisions KP, Pakistan, showed significant variations.