Title of article
Comparison the Effects of Cerium Nanoparticles (CeNP) and Cerium Oxide (CeO2) on Oxidative Toxic Stress in Human Lymphocytes
Author/Authors
Kaki ، Arastoo Department of Molecular Medicine and Genetic - Student Research Committee, Faculty of Medicine - Hamadan University of Medical Sciences , Younesi ، Hossein Department of Medical Nanotechnology - Faculty of Advanced Sciences Technology - Islamic Azad University, Pharmaceutical Sciences Branch , Hosseini ، Abdolhakim Department of Molecular Medicine and Genetic - Student Research Committee, Faculty of Medicine - Hamadan University of Medical Sciences , Mohsenzadeh ، Fariba Department of Biology, Laboratory of Plant Cell Biology - Bu-Ali Sina University , Ranjbar ، Akram Department of Toxicology and Pharmacology - School of Pharmacy - Hamadan University of Medical Sciences
From page
28
To page
32
Abstract
Background: Cerium nanoparticles (CeNP) have an interesting potential in drug delivery, gene therapy, molecular imaging and medicine. The present study focuses on the effect of exposure of cerium nanoparticles and Cerium oxide (CeO2) on oxidative stress biomarkers in human lymphocytes. Materials and Methods: Lymphocytes were incubated for 24 , 48 and 72 h at 37 °C with different concentrations (15, 30, 60 and 120 mM) of CeNP and CeO2. In lymphocytes samples evaluated oxidative stress biomarkers such as total antioxidant capacity (TAC), lipid peroxidation (LPO) and catalase activity (CAT). All data were analyzed by one way ANOVA with Tukey post hoc test. The results showed that CeNP after 24 ,48 and 72 hours caused a increase in the TAC compared to CeO2 120mg/kg group. CeNP decreased LPO level and CAT activity compared with CeO2 120mg/kg group. Results: The present study showed that after treatment for 24, 48 and 72 hours, CeO2 is able to induce oxidative toxicity in human lymphocytes. Conclusion: The results suggest that ROS intermediates are responsible for Ceinduced oxidative damage in experimental lymphocytes. Therefore, CeNP plays an important role in the alteration of oxidative injuries of human lymphocytes.
Keywords
Cerium nanoparticle , Lymphocyte , Oxidative stress
Journal title
Research in Molecular Medicine
Journal title
Research in Molecular Medicine
Record number
2761571
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