Major flavonoids identification by HPLC-MS/MS and evaluation of antiradical potential of mung bean (Vigna radiata) seed extracts in Burkina Faso

Mung bean is one of the most essential legumes in Burkina Faso due to its short maturation cycle (45 to 55 days) and its nutritional content. This work aims to contribute to a better knowledge of the bioactive compounds of the seeds of Beng Tigré, a mung bean cultivar originating from India and acclimatized in Burkina Faso. To do this, the contents of total phenolic compounds, flavonoids, and carotenoids were determined by colorimetric methods. The mineral elements were assayed by atomic absorption spectrometry after the seeds mineralization. The antiradical potential was evaluated by monitoring the disappearance of the stable radical DPPH• by UV-Visible absorption spectrophotometry at 517 nm and compared to standards. Major flavonoids were identified by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Total phenolic compounds, flavonoids, and carotenoids were 4. 356±0.162 mg GAE/g, 3. 151±0.262 mg QE/g, and 0.302±0.012 mg BCE/g, respectively. Potassium, magnesium, and calcium were, respectively, the most abundant mineral elements in the seeds: K Mg Ca Fe Zn Na Mn Cu. An antiradical activity of the Beng Tigré extract was observed with an IC50 of 209. 97±13. 55 µg of AO/g DPPH•. This activity is less effective than Gallic acid, ascorbic acid, and trolox. Finally, the HPLC-MS/MS analysis identified four compounds: naringenin 8-C-glucoside, luteolin 8-C-glucoside, apigenin 6-C-glucoside, and luteolin 7-O -rutinoside. Due to their high biological potential, these so-called bioactive compounds are the subject of growing research interest.