Green Synthesis of Ag-Se doped ZnO-Co3O4-NiO Fivenary Nanocomposite using Poly Anionic Cellulose and Evaluation of Their Anticancer and Photocatalyst Applications

This study was conducted to evaluate the photocatalytic applications and the cytotoxicity effects of Ag-Se doped ZnO-Co3O4-NiO fivenary nanocomposite synthesized using polyanionic cellulose (PAC) polymer as a stabilizer agent. Several procedures such as XRD, FESEM, FTIR, EDX, PSA, and UV-Vis were applied to investigate synthesized nanocomposite. Consistent with the FTIR spectrum, chemical bonds were seen in the structure of the nanocomposite which approved the successful synthesis of them. The XRD pattern of the synthesized nanocomposite revealed sharp diffraction peaks with high crystallinity, and pure phases of Se, Ag, ZnO, NiO, and Co3O4 were approved with XRD analysis. FESEM/PSA images indicate that nanocomposite was synthesized with an average size of 23-49 nm and relatively uniformly distributed, in addition, it has a spherical morphology. The synthesized nanocomposite exhibited excellent photocatalytic activity to methyl orange (MO) dye degradation under a UVA light source. The degradation rate of nanocomposite reached 99% within 80 min. The kinetic studies indicate that the degradation of MO dye follows a first-order kinetic model. The cytotoxicity of the nanomaterial was assessed on normal mouse fibroblast NIH3T3 and cancer mouse melanoma B16F0 cell lines with the MTT assay. The results of the MTT test revealed significant cytotoxic influences on cancer B16F0 cells (IC50 value = 258.5 µg/mL) in comparison to normal cells.