Title of article
Sorbitol MacConkey agar and wastewaters` coliphages
Author/Authors
Bedada ، Tesfaye Public Health Microbiology Research Team - Ethiopian Public Health Institute , Eshete ، Teshome School of Biomedical and Laboratory Sciences - Gondar University , Desta ، Adey Department of Microbial - Addis Ababa University , Yifat ، Fitsum Department of Microbial - Addis Ababa University , Birri ، Dagim Department of Microbial - Addis Ababa University , Tullu ، Kassu Department of Medical Laboratory Science - College of Health Sciences - Addis Ababa. University , Gebre ، Samson Public Health Microbiology Research Team - Ethiopian Public Health Institute , Dera ، Firehiwot Public Health Microbiology Research Team - Ethiopian Public Health Institute , Awoke ، Kaleab Public Health Microbiology Research Team - Ethiopian Public Health Institute , Feto ، Tatek Public Health Microbiology Research Team - Ethiopian Public Health Institute , Sima ، Waktole Public Health Microbiology Research Team - Ethiopian Public Health Institute , Deribe ، Elias Clinical Bacteriology and Mycology Reference Laboratory - Ethiopian Public Health Institute , Teklu ، Dejenie Clinical Bacteriology and Mycology Reference Laboratory - Ethiopian Public Health Institute , Irena ، Getinet Public Health Microbiology Research Team - Ethiopian Public Health Institute , Weledesemayat ، Geremew Public Health Microbiology Research Team - Ethiopian Public Health Institute , Negassi ، Tigist Public Health Microbiology Research Team - Ethiopian Public Health Institute , Garedew ، Asnake Department of Microbial, Cellular and Molecular Biology - Addis Ababa University
From page
112
To page
118
Abstract
A total of 114 samples collected from hospital wastewaters and rivers in Addis Ababa, Ethiopia were tested for non-sorbitol fermenting bacteria and coliphages. Sorbitol MacConkey agar is mainly used in the detection of E. coli O157:H7. However, other emerging diarrhoeagenic enteropathogens such as Plesiomonas shigelloides, Edwardsiella tarda, Providencia alcalifaciens, Escherichia albertii, Escherichia vulneris and Escherichia fergusonii were detected in the samples using this medium. Information for most of the emerging enteropathogens is scarce in most countries including Ethiopia. A total of 20 different genera, 38 species of non-sorbitol fermenting bacteria were isolated. Escherichia coli O157 could not be detected from any of the samples. All these backgrounds may mask the detection of Escherichia coli O157. Even if sorbitol MacConkey agar has several background limitations, different emerging diarrhoeagenic non-sorbitol fermenting bacteria were detected in the majority of the rivers and hospitals` wastewaters samples. The correlation between coliphages and non-sorbitol fermenting bacteria were not significant. As several bacteria have been isolated on sorbitol MacConkey agar medium, it is essential that the most selective laboratory techniques will be desired for outbreak investigation of E. coli O157, but other non-sorbitol fermenting enteropathogens should also be detected using sorbitol MacConkey agar in low resources countries.
Keywords
Coliphage , Emerging diarrhoeagenic bacteria , Hospitals wastewater , Sorbitol MacConkey agar , Non , sorbitol fermenting bacteria , Rivers
Journal title
Journal of Applied Research in Water and Wastewater (JARWW)
Journal title
Journal of Applied Research in Water and Wastewater (JARWW)
Record number
2765574
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