Title of article
Anti-proliferative and Apoptotic Potential of Ferula Asafoetida’s Essential Oil on Colon Cancer Cell Lines Via Inhibiting NF-κB and TNF-alpha Receptors Pathway
Author/Authors
Verma ، Shailja Institute of Science - Nirma University , Joshi ، Sanjay Department of Research and Development - The Hing Research Center , Kothari ، Vijay Institute of Science - Nirma University , Seshadri ، Sriram Institute of Science - Nirma University
From page
15
To page
21
Abstract
Background: The current investigation was conducted with the aim to elucidate and determine the anti-cancer efficacy in vitro on colon carcinoma human cell lines and to determine its probable mode of action. Methods: Anti-proliferative and apoptotic effect of various doses of essential oil from Ferula asafoetida was investigated on colon cancer cell lines i.e. CW620 and CT26. WT. The percentage cytotoxicity was determined by MTT assay. In addition, expression studies were carried out to determine the mode of action by determining the levels of NF-kB, TNF-alpha, TGF-beta and Caspases. Results: The potent anti-proliferative activity has been observed in both the cell lines. The percentage cytotoxicity is directly proportional to the increasing concentration of the essential oil. The same was also confirmed in the expression studies which shows significant upregulation of the antiapoptotic characteristics and down regulation of pro-carcinogenic factors. Conclusion: The essential oil of Ferula asafoetida shows potent anti-cancer activity and shows the role in gene regulation to attenuate the colon cancer.
Keywords
Colon Cancer , Ferula asafoetida , Essential oil , Caspases , Inflammation
Journal title
Iranian Journal of Colorectal Research
Journal title
Iranian Journal of Colorectal Research
Record number
2766487
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