Title of article
Synthesis and Characterization of Acetamidotetrazole-Grafted Magnetic Chitosan Biopolymer as a Novel Non-Virus Vector for Targeted Gene Delivery into HECK-293T Cells
Author/Authors
Mosaddegh ، Elaheh Department of New Materials - Institute of Science and High Technology and Environmental Sciences - Graduate University of Advanced Technology , Torkzadeh-Mahani ، Masoud Department of Biotechnology - Institute of Science and High Technology and Environmental Sciences - Graduate University of Advanced Technology , Hassankhani ، Asadollah Department of New Materials - Institute of Science and High Technology and Environmental Sciences - Graduate University of Advanced Technology
From page
1302
To page
1313
Abstract
This study successfully synthesized a magnetic chitosan biopolymer covalently grafted onto acetamido tetrazole and evaluated it as a high-potential biocompatible non-viruses vector. The synthesized nanocarrier was fully characterized by FT-IR spectra, TEM and FESEM images, XRD, EDX, FESEM, and DLS analyses. The loading capacity of the plasmid was optimized with the ratio N/P 3 with excellent protection of the plasmid. The nanocarrier showed high gene expression and transfection in the HECK-293T cell line without any inherent toxicity. In addition, experimental results show that the new functionalized chitosan has excellent DNA condensation, protection, and high target transfection efficiency. Therefore, the novel magnetic chitosan nanocarrier functionalized with acetamido tetrazole has shown potential uses in cancer therapy.
Keywords
Chitosan , 5 , amino , 1H , tetrazole , gene delivery , gel retardation , MTT , VSM
Journal title
Iranian Journal of Chemistry and Chemical Engineering (IJCCE)
Journal title
Iranian Journal of Chemistry and Chemical Engineering (IJCCE)
Record number
2768305
Link To Document