Isolation and Molecular Identification of Acinetobacter baumannii From Urinary Tract Infection in Diyala Province, Iraq

Background and Aim: Acinetobacter baumannii is one of the most prominent opportunistic bacterial pathogens associated with hospital-acquired infections and has been associated with antibiotic resistance. The high rates of resistance have made it difficult to choose the appropriate treatment and put the lives of infected patients at risk of death. This study aimed to isolate A. baumannii from urinary tract infections (UTI) and detect the bacterial ability to form biofilms from clinical samples. Materials and Methods: In this study, A. baumannii bacteria were isolated from several sources (UTI). The microtiter plate method revealed biofilm formation. Clinical specimens were grown on selective media. The A. baumannii was identified by classical techniques; the VITECK combined 2 system and 16S rRNA gene amplification. Results: From the 130 suspected isolates, 20 isolates were obtained from A. baumannii multidrug-resistant (MDR) and extensively drug-resistant (XDR) types. Among them, 14 (70%) were MDR and 6 (30%) were XDR types. Conclusion: Acinetobacter baumannii, Antibiotic Resistant, Biofilm, Extensively drug-resistant, Multidrug-resistant.