New Fluorescent Stains for Protein Detection in Sodium Dodecyl Sulfate–Polyacrylamide Gels

Highly sensitive new fluorescent stains for proteins in sodium dodecyl sulfate–polyacrylamide gels (SDS–PAGE) were developed by combining an environment sensitive fluorophore and a hydrocarbon tail for improved binding to SDS molecules associated with the proteins. The experimental results showed that these dyes bound to protein–SDS complexes with a concomitant emission increase upon binding.