Title of article
Cytotoxicity and Anti-Plasmodium berghei Activity of Emodin Loaded Nanoemulsion
Author/Authors
Bayat ، Fatemeh Department of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences , Motevalli Haghi ، Afsaneh Department of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences , Nateghpour ، Mehdi Department of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences , Rahimi-Esboei ، Bahman Department of Medical Parasitology and Mycology - School of Medicine - Islamic Azad University, Tonekabon Branch , Rahimi Foroushani ، Abbas Department of Epidemiology and Biostatistics - School of Public Health - Tehran University of Medical Sciences , Amani ، Amir Department of Natural Products and Medicinal Plants Research Center - North Khorasan University of Medical Sciences , Farivar ، Leila Department of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences , Sayyad Talaee ، Zahra Department of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences , Faryabi ، Aref Department of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences
From page
339
To page
348
Abstract
Background: Malaria parasites cause a tremendous burden of disease in both the tropics and subtropics areas. Growing of drugs resistance in parasites is one of the most threats to malaria control. The aim of study was to investigate the anti-malarial activity of nano-emodin isolated from Rhamnus cathartica on Plasmodium berghei in mice to evaluate parasites inhibition rate using in-vivo test. Methods: The study was conducted in the School of Public Health, Tehran University of Medical Sciences, during 2020. Nano- emodin particles were prepared from Rhamnus cathartica, and confirmed by Zeta Potential Analyzer, DLS and electron microscopy techniques. Mice were infected with P. berghei and treated by emodin nanoparticles. Parasitemia was evaluated in each group in comparison with control group. Toxicity test was done using twice the highest concentration of emodin extract on a separate group of mice and ED50 was calculated. Results: Emodin extract was significantly effective in all concentrations on D4 (P 0.05). The most effective on parasitemia was observed in 400 mg/kg of Liquid Nano-emodin and solid (non-Nano) emodin. ED50 for emodin extract was determined 220 mg/kg. Toxicity test showed no toxic effect on the subjects. Conclusion: The emodin extract is safe, lack of side effects. So, it can be used for more and longer period of time and in higher doses. Emodin extract, either in form of liquid and nanoparticle or in a solid form, has the same therapeutic effect on P. berghei in infected Balb/c mice.
Keywords
Malaria , Plasmodium berghei , Nano emodin , Rhamnus cathartica , In , vivo test
Journal title
Iranian Journal of Parasitology (IJP)
Journal title
Iranian Journal of Parasitology (IJP)
Record number
2773119
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