Developing New Antimicrobial Therapies: as a Candidate for the Treatment of Bacterial Infection with PER-1 gene Resistance to Antibiotics

The emergence of multidrug resistance in bacteria due to the overuse of antibiotics has become an important health concern in recent years, which requires the development of novel alternatives to fight against. Essential oils (EOs) are secondary metabolites that have different components and chemical compositions, which may provide promising solutions to the problem of the rising number of drug-resistant bacteria. This study aims to determine the efficacy of Eucalyptus and Geranium oil against Klebsiella pneumoniae, antibiotic-sensitive profile, and detect the molecular of the PER-1 gene by PCR method. In total 60 isolates of K. pneumoniae were collected from different hospitals in Ahvaz, (Golestan and Imam, Iran). Clevenger-type apparatus was used for oils extraction of hydro-distilled plants aerial, to determine the efficacy of essential oils. The agar well diffusion method and disc diffusion method were applied for five antibiotic discs to detect the profile of antibiotic sensitivity. Molecular detection of the PER-1 gene was done by PCR method. The results indicated that the oil extract of each plants inhibit the growth of K. pneumoniae with zones of inhibition ranging from 25 mm. Klebsiella pneumonia isolates were highly resistant to ceftazidime (83.3%), and more sensitive to Eucalyptus camaldulensis Dehnh Molecular detection of extended-spectrum β-lactamase bla PER-1 gene was recorded 12 (20%). The Geranium and Eucalyptus essential oil can be used as an alternative treatment for diseases caused by resistance to clinical isolation of K. pneumoniae.