Chrysin’s potential in ameliorating the toxic effects of Cyclophosphamide on mouse oocytes and embryos

Introduction: The study investigates the protective effects of chrysin (CR) on the quality of oocytes and embryos in rats undergoing in vitro fertilization after treatment with cyclophosphamide (CPH).  Methods: In this study, female NMRI mice were divided into five groups: I. control group, II. sham group, III. CPH group (receiving 120 mg/kg.wk of CPH intraperitoneally (IP), IV and V: CR groups receiving 5 and 10 mg/kg. day CR for four weeks. For oocyte induction, ten units of pregnant mare serum gonadotropin were injected IP after the last injection. All mice were then sacrificed by aspiration of their oocytes for further experiments. The growth of embryos was investigated using mature oocytes in vitro. Results: CR significantly increased the number of 2 cells and 4 cells after 24 and 48 hours compared to the CPH group. Groups treated with CR showed a significant increase in the expression level of the BMP-15 and GDF-9 genes in a dose-dependent manner compared to the CPH group. Conclusion: In mice, CR reduced oxidative damage and oocyte cytokine levels in ovarian tissue after CPH-induced degeneration.