Preparation of Zwitterionic Polymethacrylamide Modified with L-Lysine and Its Effect on Fibrinolytic Activity

Zwitterionic polymethacrylamides, poly(N^(alpha)-methacrylamide-L-lysine) [poly((alpha)-LysMA)] and poly(N^(epsilon)-methacrylamide-L-lysine) [poly((epsilon)-LysMA)] were prepared to examine their bioactivity by an evaluation of fibrinolytic activity and a binding assay using resonant mirror biosensor (IAsys). Poly((alpha)-LysMA) enhanced the fibrinolytic activity by plasminogen/tissue-type plasminogen activator while no enhancement was observed in the case of poly((epsilon)-LysMA). A strong interaction between Plg to poly((alpha)LysMA) was also observed by IAsys when compared with poly((epsilon)-LysMA).