Title of article
Fiber-optic Detection of DNA Denaturation for SNP Analysis
Author/Authors
Honda، Kaori نويسنده , , Kondoh، Yasumitsu نويسنده , , Kitsunai، Tokuji نويسنده , , Nishi، Katsuo نويسنده , , Tashiro، Hideo نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
-761
From page
762
To page
0
Abstract
A fiber-optic detection system for SNP analysis has been developed for monitoring the denaturation process of hybridized DNA strands on the end surface of an optical fiber. The probe DNA directly immobilized on the end surface of the fiber was hybridized to target DNA, and the denaturation process was measured. We determined the difference in melting temperature (Tm) between perfectly and SNP-matched probe-target hybrids by derivative analysis of the melting curves.
Keywords
Power requirement , prediction , Application-production research , Genetic-fuzzy system , Surface finish , grinding
Journal title
CHEMISTRY LETTERS
Serial Year
2004
Journal title
CHEMISTRY LETTERS
Record number
28493
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