Gradient-enhanced proton-fluorine NOE experiments

Uses of pulsed field gradients in the detection of 1H{19F} nuclear Overhauser effects in biological systems were examined in experiments wherein gradients were used for coherence selection and recall, as well as simply for the removal of unwanted magnetization components. Strategies for selective detection of these NOEs when the fluorine resonance is broad are proposed. Detection of 1H{19F} nuclear Overhauser effects in a carbonic anhydrasefluorinated inhibitor complex demonstrates the methods considered and shows the superiority of the "inverseʹʹapproach to detection of fluorine-proton 1H{19F} NOEs in macro-molecular systems. Copyright © 1999 John Wiley & Sons. Ltd.