An Improved Method for the Isolation of Total RNA from Malva pusilla Tissues Infected with Colletotrichum gloeosporioides

A modified RNA ex traction meth od was developed I"or funga l and plant ti ssues tha t has seve ral ad va ntages co mpared wi th ex isting. publi shed meth ods for materia ls with high co ntents of ribo nuclease. pheno lics and polysaccharides. The meth od uses guanidinium isothi ocyana te in the homogeni za ti on bufTer. and then hexadecyl trimethylammonium bromide (eTA B). so lu ble polyvinylpyrrolido ne and inso luble polyvinylpolypyrrolidoI1t;: in the ex traction butler. The proced ure consistently produced a high yield of rela tively high purity. undegntded RNA rrom a va rielY or biological muterials and should be useful for other ti ssues where RNases. phenolics and sticky polysaccha rides a rc a pro blem.