Sensory but not parasympathetic nerves are required for ocular vascular remodeling following chronic sympathectomy in rat

Choroidal vascularity increases following chronic sympathetic denervation in rats. The mechanisms of this remodeling are unclear. Since both nitric oxide and substance P/CGRP have been suggested as angiogenic factors in other targets, we hypothesized that sensory or parasympathetic nerves may also participate in ocular vascular remodeling. To test this hypothesis, sympathetic denervation was accomplished by superior cervical ganglionectomy. Sensory denervation was induced by subcutaneous injections of capsaicin on postnatal days 2 and 9, and ocular parasympathetic innervation was ablated by pterygopalatine ganglion excision on postnatal day 60. Eyes were processed and sectioned for light microscopic histomorphometry. Sympathetic denervation for 6 weeks resulted in increased choroidal thickness, vascular luminal area, numbers of large venules and large arterioles, and capillaries in the outer nuclear layer. Capsaicin pretreatment prevented sympathectomy-induced increases in choroidal thickness, vascular luminal area and large venules and large arterioles, whereas pterygopalatine ganglionectomy was without effect. Both sensory and parasympathetic denervation attenuated increases in outer nuclear layer capillaries. These studies indicate that increased choroidal vascularity noted after chronic sympathectomy requires intact sensory innervation.