Validation of a single nucleotide polymorphism genotyping assay for the human serum paraoxonase gene using electronically active customized microarrays

Objectives: Develop a microarray based genotyping assay to detect SNPs in human paraoxonase (PON I) and compare its accuracy with DNA sequencing and RFLP based assays. Design and Methods: Amplicons spanning the polymorphic regions of interest were genotyped by sequencing, RFLP, and microarray technology. Validation parameters included precision, linearity of signal response, and carryover between adjacent sites on the microarray. Results: A 100% correlation in results obtained using DNA sequencing, RFLP and microarray technology was observed. Conclusions: The microarray technology provides an accurate and reliable assay platform with applicability in high throughput genotyping studies.