Title of article
Optimizing RNA extraction yield from whole blood for microarray gene expression analysis
Author/Authors
Jian Wang، نويسنده , , John F. Robinson، نويسنده , , Hafiz M. R. Khan، نويسنده , , David E. Carter، نويسنده , , James McKinney، نويسنده , , Brooke A. Miskie، نويسنده , , Robert A. Hegele، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
4
From page
741
To page
744
Abstract
Objectives: Microarray analysis of gene expression profiles of blood leukocytes has many potential clinical and research applications.
Design and methods: We used the PAXgene Blood RNA System to prepare RNA from the whole blood of normal volunteers using two incubation times followed by gene expression profiling using the Affymetrix HU133A GeneChip.
Conclusions: Longer incubation gave a significantly higher RNA yield and samples that were satisfactory for microarray analysis, with excellent pairwise correlations between replicates.
Keywords
epidemiology , gene expression , immunology , molecular diagnosis , Metabolic disease
Journal title
Clinical Biochemistry
Serial Year
2004
Journal title
Clinical Biochemistry
Record number
482582
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