Allele-specific detection of C-1562T polymorphism in the matrix metalloproteinase-9 gene: Genotyping by MADGE

Objectives The aim was to design a method for genotyping MMP-9 C-1562T gene polymorphism that does not require RFLP PCR or sequencing. Design and methods Optimization of tetra-primer ARMS PCR was performed to obtain the highest amount of specific amplification products. Results PCR products (436 bp, 296 bp, 220 bp) were visualized on MADGE or agarose gel electrophoresis. Conclusions We designed a faster, single-step, economical method which becomes advantageous for high throughput population screening.