Real-time PCR genotyping of aldehyde dehydrogenase-2 using displacing probe

Objectives: This study aimed to develop and validate a rapid, accurate method for aldehyde dehydrogenase-2 gene (ALDH2) genotyping. Design and methods: We reported a single-tube, real-time PCR method for ALDH2 allele detection using two displacing probes. Results: The genotyping results of 136 human genomic DNA samples indicated 100% concordance between real-time PCR method and the PCR-RFLP analysis. Conclusions: The developed method could be used for routine clinical testing and high throughput genetic screening of ALDH2.