Resolution of ternary mixtures of rifampicin, isoniazid and pyrazinamide by differential pulse polarography and partial least squares method

A simple and rapid method for the joint polarographic determination of rifampicin (RIF), isoniazid (INH) and pyrazinamide (PZA) by differential pulse polarography (DPP) is described. In this paper, a partial least square (PLS) regression was used for the resolution of the overlapped polarographic signals from mixtures of the three drugs. A genetic algorithm (GA) was used to select some of the predictor variables (potentials of the polarogram). The PLS model constructed with these selected potentials also leads to satisfactory results. The procedure was successfully applied to pharmaceutical preparations and biological fluids.