Prediction of internal standards in reversed-phase liquid chromatography, IV. Correlation and prediction of retention in reversed-phase ion-pair chromatography based on linear solvation energy relationships

Three procyanidin fractions, one with shorter oligomers, one with longer oligomers and one with polymers, were obtained from Pinus sylvestris L. bark extract. Procyanidins were analyzed using reversed-phase and normal-phase high-performance liquid chromatography methods coupled with electrospray ionization mass spectrometric detection in the negative ion mode. On the grounds of mass spectral data, pine bark was found to contain procyanidins from monomers through decamers and higher polymers.