Direct Analysis of Lipids in Single Zooplankter Individuals by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

A highly sensitive method to analyze the intact lipids in a single zooplankter individual at the level of a few tenths of a microgram was developed by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) combined with a direct sampling technique. The sampling procedure involved (1) putting a zooplankter individual sample onto the MALDI sample plate, (2) cutting the sample into a few pieces by means of tweezers, (3) depositing aliquots of matrix and cationization reagent solutions on the zooplankter sample, and (4) irradiating with a N2 laser to cause MALDI. By using this technique, the mass spectra of the single zooplankter samples showed a series of ions generated from phospholipids with 34 or 36 carbons in the acyl groups and neutral lipids such as triglycerides and diacylglyceryl ethers with 50-54 carbons in their acyl and alkenyl groups. Accordingly, this method enabled us to estimate the relative quantity between "structured lipids" (phospholipids) and "storage lipids" (neutral lipids) in an individual zooplankter, which should give us a good clue to elucidate the roles of each class of lipids in its growth.