An Approach for Analysis of Protein Toxins Based on Thin Films of Lipid Mixtures in an Optical Biosensor

Biological membrane-like lipid films were deposited on the sensing surface in an optical biosensor instrument The membranes were mixtures of biologically occurring lipids. Eight surfaces were prepared, some of which contained various glycolipids as minor components. One was supplemented with membrane proteins. The binding of six protein toxins (cholera toxin, cholera toxin B subunit, diphtheria toxin, ricin, ricin B subunit, staphylococcal enterotoxin B) and of bovine serum albumin at pH 7.4 and pH 5.2 to each of the sensor surfaces was studied. Each of the seven proteins gave a distinct binding pattern. The assay is rapid and simple, with no need for reagents. The lipid sensor surface is readily regenerated after binding and very stable. The concept with mixed lipid layers and assays at different pHs gives numerous combinations and could be applicable for developing a sensor for protein toxins.