Analysis of oxidized heme proteins and its application to multiple antioxidant protection

Oxidation in tissues and homogenates can be determined by the analysis of oxidized heme proteins. Oxidation of heme proteins can be measured by spectral changes and the deconvolution of the spectra of mixtures of heme proteins by a spreadsheet heme spectra analysis program (HSAP), incorporating the spectra of the individual pure heme proteins. HSAP also is used to analyze the spectra of mixtures of heme proteins found in the literature. HSAP is applied in measuring the protective effects in rats of multiple antioxidants suitable for use in humans for protection against diseases.