Proton Inhibition of Transient Outward Potassium Current in Rat Ventricular Myocytes

Acidosis elicited during myocardial ischemia is a significant pathophysiological condition markedly affecting the electrical and contractile properties of heart muscle.We examined the effects of protons on K+channel activity in rat ventricular myocytes by recording transient outward (Ito) and inward rectifier (IK1) K+currents using the whole cell, voltage clamp technique. Proton concentration was controlled by independently varying the pH of HEPES-buffered external (pHo) or pipette (pHp) solutions. Mean Itodensity in myocytes preconditioned in acidic external solution (pHo6.0) for 15–20 min was significantly less than control cells equilibrated at physiological pHo. In contrast, IK1was not changed during this period of acidosis. External acidification did not decrease Itowhen initiated after intracellular dialysis with standard pHp7.2. However, when myocytes were dialyzed with acidic pHp, Itodensity was significantly less than control, while alkaline pHphad little effect. Despite marked reduction in current density produced by low pHpsolutions, steady-state activation and inactivation parameters of Itowere not significantly altered. In addition, the reversal potential of this current, kinetics of inactivation, and recovery from inactivation were not significantly affected by acidic or alkaline pHpsolutions. Acidic pHpalone did not change IK1density compared with control, but when combined with Na+/H+exchange blockade with 5-(N,N-dimethyl)-amiloride or Na+-free external solution, IK1density was significantly reduced. Our data suggest that protons inhibit Itopredominantly from the intracellular side of the channel, possibly by altering its conductance or gating properties. Moreover, intracellular protons differentially affect Itoand IK1channels, with the former exhibiting greater sensitivity for a given level of acidosis.