X-ray fluorescence analysis of bromine for the estimation of extracellular water

Three methods for extracellular water (ECW) estimation via analysis of the corrected bromine space (CBS) are presented. The methods are based on the application of stable bromine as an indicator and X-ray fluorescence analysis (XRF). The content of stable bromine can be determined in samples of ulna vein blood and daily urine (the first method) as well as in microsamples of finger blood (the second method) and mixed non-stimulated saliva (the third method). The precision and accuracy of the methods were compared using the results of both repeated analysis and CBS estimation by different procedures including the routine method with 82Br as indicator. A device for XRF with a Si(Li) detector and 109Cd sources was developed for bromine determination in biological fluids.