In vitro evaluation of the effect of freshly mixed amalgam and gallium-based alloy on the viability of primary periosteal and osteoblast cell cultures

The effect of freshly mixed specimens of a gallium-based alloy Galloy® and a high copper spherical amalgam Tytin® on the viability of primary periosteal and osteoblast cells was investigated. The cells were grown from the parietal bones of 2–3 day old Albino Wistar rats and were seeded in multi-well plates and exposed to the test materials for 1 or 6 days. The number of viable cells in each test group was determined using the Trypan blue dye exclusion test and compared with the controls. The area of cell death around the test specimens was also measured. Statistical analysis (ANOVA, Tukeyʹs pairwise comparisons) showed a significant effect of the test materials on cell viability (P<0.01). The viability of cultures containing Tytin was significantly lower than cultures with Galloy and the controls (P<0.05). The viability of cultures containing Galloy was significantly lower (P<0.05) than controls except for periosteal cells after 1 day. The culture media was analysed using atomic absorption spectrophotometry (AAS) for metal ion content. The results suggested that mercury (Hg) was the main element released from the dental amalgam followed by copper (Cu) and silver (Ag) whilst gallium (Ga) was the main element released from the gallium-based alloy followed by indium (In), Ag and Cu.