Interaction of cyanine dyes with nucleic acids. XX. New methods for the preparation of fluorescent probes based on benzothiazol-4-[2,6-dimethylpyridinium] cyanine dyes

Two new methods of preparing fluorescent dyes containing affinity-modifying groups for nucleic acid detection are proposed. One method of preparation is based on the reaction of the pyrylium monomethine cyanine dye 2-(2,6-dimethyl-pyran-4-ilidenmethyl)-3-methylbenzothiazolium perchlorate with primary amines. The second method of preparation involves the amidation of carboxy substituted dyes with appropriate amines.