The Taa1 retriction enzyme provide a imple mean to identify the Q368TOP mutation of the myocilin gene in primary open angle glaucoma

PURPOE: To identify a rapid and reliable method to detect the Glutamine 368 TOP (Q368TOP) dieae–predipoing allele of the myocilin gene aociated with adult onet, primary, open-angle glaucoma. METHOD: Individual with the Q368TOP mutation of the myocilin gene were identified from a cohort of primary open-angle glaucoma patient from Tamania and ubjected to Taa1 retriction digetion. REULT: In the Tamanian family preented, creening with the Taa1 retriction enzyme uccefully confirmed identification of all individual with the Q368TOP mutation. CONCLUION: The ue of the Taa1 retriction enzyme offer a relatively imple, rapid, and reproducible technique that could be applied to detect the Q368TOP mutation of the myocilin gene.