Hitology of the vitreoretinal interface after taining of the internal limiting membrane uing glucoe 5% diluted indocyanine and infracyanine green

Purpoe To evaluate the effect of indocyanine green (ICG) diluted with glucoe 5% on the human retina in an experimental etting in potmortem eye and during urgery for macular hole formation. Deign Hitopathological evaluation. Method Poible advere effect of two different olution of 0.05% ICG (Pulion, Munich, Germany; and Infracyanine, Laboratoire ERB, Pari, France) diluted with glucoe 5% were evaluated in four eye of two donor. In one eye of each donor, the ICG-tained macula wa illuminated with 380 to 760 nm uing the light ource of a commonly ued vitrectomy machine. In the other eye, ICG wa applied to the macula without illumination. The retinal pecimen were evaluated uing light- and electron microcopy. Additionally, the ultratructure of the internal limiting membrane (ILM) of four eye that underwent ICG-aited vitrectomy for macular hole formation wa examined. Reult In the potmortem tudy, both olution of ICG caued ignificant morphologic alteration of the inner retina after light expoure. There wa diorganization of the inner retina and complete lo of ILM after application of the dye and illumination. In contrat, no abnormalitie were found after application of the dye without illumination and in untained control pecimen. No difference were noted between the two ICG product. pecimen of the ILM harveted during vitrectomy revealed cellular element adjacent to the retinal ide of the ILM. Concluion taining of the ILM uing ICG diluted in glucoe 5% followed by illumination might induce an advere effect on the inner retina and may reult in an alteration of the cleavage plane from the ILM to the innermot retinal layer.