Photodynamic Action of Indocyanine Green and Trypan Blue on Human Len Epithelial Cell In Vitro

Purpoe The purpoe of thi tudy wa to evaluate the toxicity and photodynamic activity of indocyanine green (ICG) and trypan blue (TryB) on cultured human lenepithelial cell (LEC). Deign Experimental tudy. Method Len epithelial cell viability wa aeed after treatment with ICG and TryB concentration ranging from 0.025 to 5.0 mg/ml, and expoure to 806 nm diode laer. Reult At ICG concentration below 0.5 mg/ml, there wa ≥75% cell viability; at higher ICG concentration there wa doe-dependent cytotoxicity in addition to lo of cellular viability due to ICG photoenitization. TryB had little cytotoxicity to the LEC: >80% cell were viable irrepective of the dye concentration or laer treatment. Concluion Thee data indicate that ICG may have application a a photoenitizer in the elective eradication of reidual LEC after cataract urgery to reduce the incidence of poterior capule opacification.