Application of fermentor technology in production of diphtheria toxin

The present study shows the production of diphtheria toxoid in fermentor as compared to old static method of production. With using 45l glass fermentor which has the provision for growth of the organisms under controlled conditions with regard to temperature, pH, aeration and addition of maltose which can be automatically monitored and maintain microorganism growth toxin production and recording all of the conditions during growth. We succeeded to produce diphtheria toxin with 115 Lf (Rammon flucolation titer) within 40h time period in a minimum possible space, then the toxin was detoxified with formaldehyde, concentrated and purified and finally quality controlled and compared with toxin produced with static method. Fermentor production showed a remarkable increase in volume, titer and immunogenecity in a shorter period of time, lower contamination and minimum labor work and requirements. Therefore this technique can be industrially used for production of diphtheria Toxin (DT) and Diphtheria Pertussis Toxin (DPT) vaccines.