Micropropagation of Nerium Oleander through the Immature Pods

Oleander (Nerium oleander L.) is a vegetatively propagated ornamental plant valued for its evergreen foliageand showy terminal flower clusters that are available in different colours. Oleander is cultivated recently as aflowering pot plant and therefore abundant propagation plant material for commercial use is of great importance. This species also produces secondary metabolites (Paper & Franz 1989), some of which are of pharmacologicalinterest. In vitro culture of plants has gained importance during recent years because, besides other application, this technique can be used for the rapid multiplication of some plants (Tisserat 1987). As far as we are aware, there are no published reports about micropropagation of Nerium oleander and the aim of the present work wasto determine the culture conditions for micropropagation of this plant. To induce callus formation from pods of Nerium oleander,and study the in vitro growth conditions of calli. Study the specific growth factor for root and shoot induction, and transplantation and acclimatization of explantsand isolation of the DNA from explants. Standardization of micropropagation protocol of Nerium oleander. In the case of Nerium oleander, the micropropagation technique from pods were established. We also raisedplantlets from the callus and also subjected the DNA for analysis and done RAPD to find the difference betweenthe normal and in vitro plants, the result is no difference-this shows that the plants are identical. Standardizationof surface sterilization protocol, aseptic culture initiation,establishment and multiplication, suitable media forrooting and a suitable protocol for hardening in order to achieve quality transplant.Optimizitation of medium forcallus induction, maintenance and regeneration